Enhancement of murine coronavirus replication by severe acute respiratory syndrome coronavirus protein 6 requires the N-terminal hydrophobic region but not C-terminal sorting motifs.
Identifieur interne : 003717 ( Main/Exploration ); précédent : 003716; suivant : 003718Enhancement of murine coronavirus replication by severe acute respiratory syndrome coronavirus protein 6 requires the N-terminal hydrophobic region but not C-terminal sorting motifs.
Auteurs : Jason Netland [États-Unis] ; Debra Ferraro ; Lecia Pewe ; Heidi Olivares ; Thomas Gallagher ; Stanley PerlmanSource :
- Journal of virology [ 0022-538X ] ; 2007.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Protéines virales.
- physiologie : Protéines virales.
- Animaux, Infections à coronavirus, Interactions hydrophobes et hydrophiles, Motifs d'acides aminés, Mutation, Protéines virales, Réplication virale, Souris, Virus du SRAS.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Viral Proteins.
- chemistry : SARS Virus.
- chemical , genetics : Viral Proteins.
- chemical , physiology : Viral Proteins.
- Amino Acid Motifs, Animals, Coronavirus Infections, Hydrophobic and Hydrophilic Interactions, Mice, Mutation, Virus Replication.
Abstract
Severe acute respiratory syndrome coronavirus encodes several accessory proteins of unknown function. We previously showed that one such protein, encoded by ORF6, enhanced the growth of mouse hepatitis virus in tissue culture cells and in mice. Protein 6 consists of an N-terminal hydrophobic peptide and a C-terminal region containing intracellular protein sorting motifs. Herein, we show that mutation of the hydrophobic region but not the sorting motifs affected the ability of protein 6 to enhance virus growth. Collectively, these results support the notion that the 6 protein interacts with membrane-bound viral replication or assembly machinery to directly enhance virus replication and virulence in animals.
DOI: 10.1128/JVI.01308-07
PubMed: 17670827
Affiliations:
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Ferraro</name></author><author><name sortKey="Pewe, Lecia" sort="Pewe, Lecia" uniqKey="Pewe L" first="Lecia" last="Pewe">Lecia Pewe</name></author><author><name sortKey="Olivares, Heidi" sort="Olivares, Heidi" uniqKey="Olivares H" first="Heidi" last="Olivares">Heidi Olivares</name></author><author><name sortKey="Gallagher, Thomas" sort="Gallagher, Thomas" uniqKey="Gallagher T" first="Thomas" last="Gallagher">Thomas Gallagher</name></author><author><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17670827</idno><idno type="pmid">17670827</idno><idno type="doi">10.1128/JVI.01308-07</idno><idno type="wicri:Area/PubMed/Corpus">001D55</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001D55</idno><idno 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uniqKey="Netland J" first="Jason" last="Netland">Jason Netland</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology, University of Iowa, BSB 3-712, Iowa City, IA 52242, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology, University of Iowa, BSB 3-712, Iowa City, IA 52242</wicri:regionArea><placeName><region type="state">Iowa</region><settlement type="city">Iowa City</settlement></placeName><orgName type="university">Université de l'Iowa</orgName></affiliation></author><author><name sortKey="Ferraro, Debra" sort="Ferraro, Debra" uniqKey="Ferraro D" first="Debra" last="Ferraro">Debra Ferraro</name></author><author><name sortKey="Pewe, Lecia" sort="Pewe, Lecia" uniqKey="Pewe L" first="Lecia" last="Pewe">Lecia Pewe</name></author><author><name sortKey="Olivares, Heidi" sort="Olivares, Heidi" uniqKey="Olivares H" first="Heidi" last="Olivares">Heidi Olivares</name></author><author><name sortKey="Gallagher, Thomas" sort="Gallagher, Thomas" uniqKey="Gallagher T" first="Thomas" last="Gallagher">Thomas Gallagher</name></author><author><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name></author></analytic><series><title level="j">Journal of virology</title><idno type="ISSN">0022-538X</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Motifs</term><term>Animals</term><term>Coronavirus Infections</term><term>Hydrophobic and Hydrophilic Interactions</term><term>Mice</term><term>Mutation</term><term>SARS Virus (chemistry)</term><term>Viral Proteins (chemistry)</term><term>Viral Proteins (genetics)</term><term>Viral Proteins (physiology)</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Infections à coronavirus</term><term>Interactions hydrophobes et hydrophiles</term><term>Motifs d'acides aminés</term><term>Mutation</term><term>Protéines virales ()</term><term>Protéines virales (génétique)</term><term>Protéines virales (physiologie)</term><term>Réplication virale</term><term>Souris</term><term>Virus du SRAS ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Protéines virales</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Motifs</term><term>Animals</term><term>Coronavirus Infections</term><term>Hydrophobic and Hydrophilic Interactions</term><term>Mice</term><term>Mutation</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Infections à coronavirus</term><term>Interactions hydrophobes et hydrophiles</term><term>Motifs d'acides aminés</term><term>Mutation</term><term>Protéines virales</term><term>Réplication virale</term><term>Souris</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome coronavirus encodes several accessory proteins of unknown function. We previously showed that one such protein, encoded by ORF6, enhanced the growth of mouse hepatitis virus in tissue culture cells and in mice. Protein 6 consists of an N-terminal hydrophobic peptide and a C-terminal region containing intracellular protein sorting motifs. Herein, we show that mutation of the hydrophobic region but not the sorting motifs affected the ability of protein 6 to enhance virus growth. Collectively, these results support the notion that the 6 protein interacts with membrane-bound viral replication or assembly machinery to directly enhance virus replication and virulence in animals.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Iowa</li></region><settlement><li>Iowa City</li></settlement><orgName><li>Université de l'Iowa</li></orgName></list><tree><noCountry><name sortKey="Ferraro, Debra" sort="Ferraro, Debra" uniqKey="Ferraro D" first="Debra" last="Ferraro">Debra Ferraro</name><name sortKey="Gallagher, Thomas" sort="Gallagher, Thomas" uniqKey="Gallagher T" first="Thomas" last="Gallagher">Thomas Gallagher</name><name sortKey="Olivares, Heidi" sort="Olivares, Heidi" uniqKey="Olivares H" first="Heidi" last="Olivares">Heidi Olivares</name><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name><name sortKey="Pewe, Lecia" sort="Pewe, Lecia" uniqKey="Pewe L" first="Lecia" last="Pewe">Lecia Pewe</name></noCountry><country name="États-Unis"><region name="Iowa"><name sortKey="Netland, Jason" sort="Netland, Jason" uniqKey="Netland J" first="Jason" last="Netland">Jason Netland</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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